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PromARK™

IHC Detection and Components for Use With Animal Tissue

Biocare’s PromARK micro-polymer detection systems are designed specifically for use on animal tissues. The micro-polymer technology provides superior sensitivity and minimum cross-reactivity to endogenous mouse and rat IgG. The Promark product line includes specially formulated heat-retrieval solutions, blocking agents, and one- and two-step detection reagents.

  • High staining sensitivity and specificity
  • Minimum cross-reactivity to endogenous IgG with proprietary blocking reagents
  • Elimination of endogenous biotin
  • Reduced number of IHC steps
  • Use with formalin-fixed, paraffin-embedded tissues, floating sections, frozen sections and cell culture preparations
  • Highly effective ancillary reagents
  • Suitable for both manual and automated techniques

Animal tissues contain endogenous immunoglobulins that produce significantly high levels of background staining when standard anti-mouse/anti-rabbit detection systems are employed. Biocare provides micro-polymer detection systems for use with primary antibodies from various sources (mouse, rat, rabbit, goat) on a range of tissues (mouse, rat, canine, feline, bovine, equine, porcine, ovine) which result in reduced background staining and higher sensitivity.

Mouse-on-Mouse Avidin-Biotin Biotinylation Double Stain Kit: Mouse PCNA + Rabbit CD3 on mouse spleenRabbit-on-Rodent: Rabbit CD3 on Mouse Spleen

PromARK Micro-polymer Detection Systems Reference Table

PromARK Micro-polymer Primary Ab Species Tissue Species Label Blocking Reagent Retrieval Reagent
Mouse-on-Farma Mouse Bovine, Equine, Porcine & Ovine HRP or AP Background Punisher Reveal / Diva / Borg
Rabbit-on-Farma Rabbit Bovine, Equine, Porcine & Ovine HRP or AP Background Punisher Reveal / Diva / Borg
Mouse-on-Canine Mouse Canine & Feline HRP or AP Background Punisher Reveal / Diva / Borg
Rabbit-on-Canine Rabbit Canine & Feline HRP or AP Background Punisher Reveal / Diva / Borg
Goat-on-Rodent Goat Mouse HRP or AP Rodent Block M Rodent Decloaker
Mouse-on-Mouse Mouse Mouse HRP or AP Rodent Block M Rodent Decloaker
Rabbit-on-Rodent Rabbit Mouse HRP or AP Rodent Block M Rodent Decloaker
Rat-on-Mouse Rat Mouse HRP or AP Rodent Block M Rodent Decloaker
Goat-on-Rodent Goat Rat HRP or AP Rodent Block R Rodent Decloaker
Mouse-on-Rat Mouse Rat HRP or AP Rodent Block R Rodent Decloaker
Rabbit-on-Rodent Rabbit Rat HRP or AP Rodent Block R Rodent Decloaker
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